unc-108 (Rab2) mutations suppress the hyperactivity and improve the growth of goa-1(sa734). (A) Representative photos with genotypes indicated. Images are scaled identically. Wild-type length is ∼1 mm. (B) unc-108 mutations confer sluggish locomotion in (+) and goa-1 mutant backgrounds. Spontaneous (unstimulated) locomotion rates of N2 (wild type) compared with the indicated genotypes are shown. The last three bars are site of action rescue experiments in which unc-108 loss-of-function mutants contain transgenes that express the unc-108 cDNA from the unc-108 promoter, panneuronally (rab-3 promoter), or in body wall muscle (myo-3 promoter). Error bars are SEMs of 10 animals each. (C) unc-108 single mutants and unc-108 goa-1 double mutants are sluggish even in response to a harsh stimulus. Locomotion responses of N2 compared with the indicated genotypes at the indicated intervals after a defined mechanical stimulus. Error bars are SEMs of 30 animals each. (D) unc-108 and unc-31 mutations improve the growth rate of goa-1 null mutants. Error bars are the SEMs of three populations of animals plated as first-stage larvae and allowed to grow and produce progeny for 96 h at 20°C.