Localization of myosin-GFP in a cell with a radially symmetrical monopolar spindle. Monopolar anaphases were induced by RNAi depletions of the Kinesin-5 motor (Klp61F in Drosophila) and BubR1. This cell, imaged by TIRF microscopy, localizes myosin-GFP symmetrically at its periphery at early anaphase (4.3 min) but then redistributes the myosin toward the center of the cortical footprint more slowly (14.7 and 18 min). See Video 10. Bar, 10 µm.