Localization of microtubules and myosin at the cortex during anaphase in wild type and cells depleted of Kinesin-6 by RNAi. mCherry-tubulin and myosin-GFP were imaged by dual-color TIRF microscopy. The insets in the wild-type cell show enlarged views of stable microtubule bundles that appear at the equatorial cortex within the TIRF illumination field. The interior, bipolar mitotic spindle is not visible by TIRF imaging but is aligned and extends perpendicular to the band of equatorial myosin accumulation (as in Fig. 1). Depletion of the Kinesin-6 motor by RNAi prevents both the accumulation of myosin-GFP at the equator and loss at the poles and reduces the number of equatorial microtubule bundles during anaphase. Although myosin does not accumulate at the equator, anaphase is noted by the increase in the number of microtubules that reach the cortex in the Kinesin-6 RNAi cells. Kinesin-6 RNAi cells also tend to have a more homogeneous pattern of myosin at the cortex during metaphase (compare 0 s time points). See Videos 2 (wild type) and 6 (Kinesin-6 RNAi). Bars, 10 µm.