Time course of myosin increase at the equator (red) and decrease at one of the poles (blue) in four representative cells. Using ImageJ, a 3.17 × 3.17-µm area (boxes shown in the inset) was measured for its mean intensity over time at the cell equator or the pole over an image stack. Background intensity in a nearby area outside of the cell was subtracted from the above measurement. Fluorescence intensity is in arbitrary units.