Redistribution of myosin-GFP during anaphase in Drosophila S2 cells as observed by TIRF microscopy. (A) A schematic diagram showing the experimental system of a Drosophila S2 cells adsorbed onto a Con A–coated coverslip and imaged using total internal reflection laser illumination. (B) A time sequence showing myosin-GFP imaged by TIRF (left), mCherry-tubulin imaged by wide field fluorescence (center), and a merge of the two images (right). The insets at 36 and 74 sec show enlarged views of a region at the equator where individual myosin rods (likely bipolar filaments) suddenly appear during early anaphase. For dynamics, see Video 1. Bar, 10 µm.