Figure 4.
Figure 4. Cortactin tyrosine phosphorylation and cofilin are important for barbed end formation at invadopodium precursors. (A and B) Barbed end assay in response to EGF with cortactin WT or 3YF cells, treated with cortactin siRNA. (A) Representative images and (B) quantification of barbed end intensity in response to EGF at invadopodium precursors normalized to WT at 0 s. Bar, 10 μm. P values are compared with WT at the same time point. Throughout the figure, n = number of invadopodium precursors; three independent experiments: WT 0 (39), 3YF 0 (24), WT 60 (255), 3YF 60 (103), WT 120 (192), 3YF 120 (171). (C) Representative images and (D) quantification of barbed ends in response to EGF in ctrl vs. cofilin siRNA treated cells normalized to 0 s. Bar, 10 μm. P values are compared with ctrl siRNA at the same time point. n: ctrl siRNA 0 (33), 60 (42), 120 (60) and cofilin siRNA 0 (57), 60 (49), 120 (110).

Cortactin tyrosine phosphorylation and cofilin are important for barbed end formation at invadopodium precursors. (A and B) Barbed end assay in response to EGF with cortactin WT or 3YF cells, treated with cortactin siRNA. (A) Representative images and (B) quantification of barbed end intensity in response to EGF at invadopodium precursors normalized to WT at 0 s. Bar, 10 μm. P values are compared with WT at the same time point. Throughout the figure, n = number of invadopodium precursors; three independent experiments: WT 0 (39), 3YF 0 (24), WT 60 (255), 3YF 60 (103), WT 120 (192), 3YF 120 (171). (C) Representative images and (D) quantification of barbed ends in response to EGF in ctrl vs. cofilin siRNA treated cells normalized to 0 s. Bar, 10 μm. P values are compared with ctrl siRNA at the same time point. n: ctrl siRNA 0 (33), 60 (42), 120 (60) and cofilin siRNA 0 (57), 60 (49), 120 (110).

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