Rescue with mechanochemically compromised versions of myoJ results in corresponding changes in CV membrane dynamics. Shown are individual frames from the three movies presented in Video 8 that demonstrate the typical behavior at the ventral surface of CV bladders after water discharge in myoJ-null cells (bound using poly-lysine and imaged with GFP-dajumin) (A1–A8), myoJ-null cells complemented with GFP-myoJ G488A (B1–B8), and myoJ-null cells complemented with GFP-myoJ IQ1,2 (C1–C8). We note that we did not observe full fusion when myoJ-null cells were bound to poly-lysine–coated glass (Heuser, 2006), presumably because we used a lower concentration of poly-lysine. (D) Quantitative data for the speed of tubules moving along the cortex in the indicated strains. The values for null cells rescued with GFP-myoJ IQ1,2 or GFP-myoJ IQ1,6 were both significantly different from both controls (WT cells imaged with GFP-dajumin and null cells rescued with GFP-myoJ) (P < 0.001 in every pairing; Mann-Whitney). Bars: (A8, B8, and C8) 2 µm.