WT cells depleted of F-actin or overexpressing myoJ's cargo-binding globular tail domain exhibit the same defect in CV membrane distribution as do myoJ-null cells. Shown are a transmitted/overlaid image (A) and the corresponding fluorescence image (B) of a field of WT cells expressing GFP-dajumin that were incubated for 20 min with 10 µM cytochalasin A. C–E show a cytochalasin-treated cell expressing GFP-dajumin (D) that was stained for microtubules (C) (E; overlaid image). F and G show a fluorescent image of a WT cell expressing GFP-myoJ GTD (F) and the corresponding transmitted/overlaid image (G). H–J show several WT cells that were expressing GFP-myoJ-GTD (H) and that were agar overlaid, fixed, and stained with the anti-proton pump antibody (I) (J; overlaid image). Bars: (B) 5 µm; (E) 4 µm; (G and J) 3 µm.