CV membrane dynamics in myoJ-null cells and in WT cells depleted of either F-actin or microtubules. Shown are individual frames from the three movies presented in Video 5 that demonstrate the typical behavior of CV membranes in myoJ-null cells (A1–A6), WT cells treated with cytochalasin (B1–B6), and WT cells treated with NZ (C1–C6). The meanings of the arrowheads are described in the text (note that the top arrowhead in A5 and A6 points out an additional CV tubule emanating from the central mass of CV membranes). Although not apparent in B1–B6, the continued incubation of WT cells in the presence of cytochalasin results in the progressive accumulation of GFP-dajumin in the plasma membrane, due presumably to a shift from kiss-and-run to full membrane fusion during bladder discharge events (Heuser, 2006). Bar: (A6) 4 µm.