The central aggregate of CV membranes in myoJ-null cells is focused at the MTOC. Shown are a WT cell (A–C) and a myoJ-null cell (D–F) that were expressing GFP-dajumin (B and E) and that were agar overlaid and stained for α-tubulin (A and D). (C and F) Corresponding overlaid image. Also shown are two myoJ-null cells (G) that were expressing GFP-dajumin (H) and that were stained for γ-tubulin (I). J shows the overlaid image. The cell in the middle of the image has two closely spaced centrosomes. Bars: (C and E) 3 µm; (J) 5 µm.