Correlations between FA morphological features in control cells. (a) Scatter plots of FA morphological parameters from two different control wells (one high-resolution image per well) showing the correlation between the following FA morphological parameters: FA area, FA mean intensity (A.U., arbitrary units), and FA length. The mean correlation value (R) is shown. (b) A typical image of control cells (RISC Free), segmented in order to define FA objects and calculate their parameters. The centers of mass of the FAs are marked by crosses, color-coded according to the magnitude of the designated feature (area, mean intensity, and length). Red, high; yellow, medium; blue, low. Bar, 10 µm.