F-actin is required for Cno cortical localization. (A) Actin (Act) cosedimentation assays of GST-CnoCT, GST-αcat, and GST as a negative control are shown. S, supernatant; P, high speed pellet. (B–B” and D–D”’) DMSO-treated controls. (B–B”) DEcad at apical and basal junctions. Cno only at apical junctions. (C–C” and E–E”’) Cytochalasin treated. (C–C”) After depolymerization, DEcad all along lateral cortex. Cno cytoplasmic and nuclear. (D–D”’) Normal DEcad, Cno, and actin localization. (E–E”’) Actin depolymerized, some residual cortical actin in cells at left (arrows). DEcad remains cortical. Cno lost from cortex (arrowheads) except where residual cortical actin remains. Bars, 10 µm.