Figure 9. Rap1 but not AJs or Ed are... | Rockefeller University Press

Figure 9.

$Figure 9. Rap1 but not AJs or Ed are required for apical Cno recruitment. (A) Purified Cno PDZ domain incubated with GST or GST fused to C-terminal seven amino acids of DEcad. Input, 1% of load; bound, 10% of bound fraction. (B) Embryonic extracts immunoprecipitated with anti-Arm. Input, unbound (UN), and immunoprecipitation (IP) fractions immunoblotted with the indicated antibodies. (C–J’) Antigens and genotypes are indicated. Apical surface is shown except for insets in D and F, which show cross sections. (C–H’) Late cellularization. (I–J’) Early gastrulation. (C–F”) Removing AJs (C–D”; arm043A01) or Ed (E–F”; edF72) does not affect Cno localization. (G–J’) Removing Rap1 reduces cortical Cno. WT, wild type. Bars, 10 µm.$

Rap1 but not AJs or Ed are required for apical Cno recruitment. (A) Purified Cno PDZ domain incubated with GST or GST fused to C-terminal seven amino acids of DEcad. Input, 1% of load; bound, 10% of bound fraction. (B) Embryonic extracts immunoprecipitated with anti-Arm. Input, unbound (UN), and immunoprecipitation (IP) fractions immunoblotted with the indicated antibodies. (C–J’) Antigens and genotypes are indicated. Apical surface is shown except for insets in D and F, which show cross sections. (C–H’) Late cellularization. (I–J’) Early gastrulation. (C–F”) Removing AJs (C–D”; arm^043A01) or Ed (E–F”; ed^F72) does not affect Cno localization. (G–J’) Removing Rap1 reduces cortical Cno. WT, wild type. Bars, 10 µm.