Cno is enriched at tricellular junctions with a subpool of actin. Wild type, antigens are indicated. (A–F”) Surface views are shown. (G–I”) Cross sections are shown. (A–B”) Cellularization is shown. (A–A”) Apically, Cno colocalizes with Arm and Ed (inset) in spot AJs, with enrichment at tricellular junctions (arrowheads). (B–B”) 2 µm more basal, Cno is strongly enriched at tricellular junctions relative to Arm (arrowheads). (C–D”) Mid (C–C”) to late (D–D”) gastrulation. Cno, Arm, and Ed (insets) form belt AJs. Cno remains enriched at tricellular junctions (arrowheads). (E–F”) Cno localizes with a subpool of actin at tricellular junctions (arrowheads) during cellularization (E–E”) and gastrulation (F–F”; E” [inset], actin visualized with moe-GFP). (G) Cno is already apical in the syncytial embryo (arrow). (H–H”) Cno colocalizes with DEcad in apical AJs (arrows) but not basal junctions (arrowheads). (I–I”) Gastrulation. Cno tightly localized at AJs with Arm and Ed. The inset shows Cno and Ed channels alone. Bars, 10 µm.