Figure 3.
Figure 3. GRASP65 must be present in opposing mitochondrial membranes. G65-GFP-ActA–transfected HeLa cells were seeded together with either mCherry-ActA (A–C) or G65-mCherry-ActA (D–F) transfected HeLa cells, and the cells were fused with polyethylene glycol. Cycloheximide was added and after 3 h the heterokaryons, without BFA treatment, were fixed and visualized. Bar, 10 µm.

GRASP65 must be present in opposing mitochondrial membranes. G65-GFP-ActA–transfected HeLa cells were seeded together with either mCherry-ActA (A–C) or G65-mCherry-ActA (D–F) transfected HeLa cells, and the cells were fused with polyethylene glycol. Cycloheximide was added and after 3 h the heterokaryons, without BFA treatment, were fixed and visualized. Bar, 10 µm.

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