Figure 9.
Figure 9. GABA promoted migration of NG2 cells in tissue explant preparations. (A) Schematic diagram of the tissue explant migration assay. Four SVZa explants (open circles) were cultured on one poly-d-lysine–coated coverslip. 1 or 2 d after culturing, GABA (50 mM, 50 µl) with or without various inhibitors was added to the well on top of the agarose block (8 mm on a side) sitting in the center of the coverslip to create a concentration gradient of the drugs in the medium surrounding the agarose block. (B) Images of anti-NG2 immunostaining showing the asymmetric or symmetric distribution of NG2 cells migrating from the edge of the explant with or without GABA added to the agarose block. Arrows indicate the direction of the agarose block. Bar, 50 µm. (C) Accumulated distribution of migrated NG2 cells in the proximal and distal quadrants of the SVZa explant in the presence of a GABA-containing agarose block as shown in B. x axis, final distance of migrated NG2 cells from the edge of the explants. y axis, percentage of NG2 cells that migrated further than the distance indicated on the x axis. (D) Averaged ratio (proximal/distal quadrants) of NG2 cells migrating out of explants under various conditions. The number of migrated NG2 cells in the proximal quadrant of the explant was normalized with that in the distal quadrant of the same explant. The number associated with each column represents the number of explants examined. *, P < 0.05 compared with control group. (E) Averaged ratio (proximal/distal quadrants) of the mean migration distance of NG2 cells from the edge of explants under various conditions. The mean migration distance of NG2 cells in the proximal quadrant of the explant was normalized with that in the distal quadrant of the same explant. Data were analyzed from the same explants as shown in D. **, P < 0.01 compared with control group. (F) Averaged ratio (proximal/distal quadrants) of the total migration distance of NG2 cells from the edge of SVZ explants under various conditions. The summed migration distance of all the NG2 cells in the proximal quadrant of the explant was normalized with that in the distal quadrant of the same explant. Data were analyzed from the same explants as shown in D. **, P < 0.01 compared with the control group. Error bars represent mean ± SEM.

GABA promoted migration of NG2 cells in tissue explant preparations. (A) Schematic diagram of the tissue explant migration assay. Four SVZa explants (open circles) were cultured on one poly-d-lysine–coated coverslip. 1 or 2 d after culturing, GABA (50 mM, 50 µl) with or without various inhibitors was added to the well on top of the agarose block (8 mm on a side) sitting in the center of the coverslip to create a concentration gradient of the drugs in the medium surrounding the agarose block. (B) Images of anti-NG2 immunostaining showing the asymmetric or symmetric distribution of NG2 cells migrating from the edge of the explant with or without GABA added to the agarose block. Arrows indicate the direction of the agarose block. Bar, 50 µm. (C) Accumulated distribution of migrated NG2 cells in the proximal and distal quadrants of the SVZa explant in the presence of a GABA-containing agarose block as shown in B. x axis, final distance of migrated NG2 cells from the edge of the explants. y axis, percentage of NG2 cells that migrated further than the distance indicated on the x axis. (D) Averaged ratio (proximal/distal quadrants) of NG2 cells migrating out of explants under various conditions. The number of migrated NG2 cells in the proximal quadrant of the explant was normalized with that in the distal quadrant of the same explant. The number associated with each column represents the number of explants examined. *, P < 0.05 compared with control group. (E) Averaged ratio (proximal/distal quadrants) of the mean migration distance of NG2 cells from the edge of explants under various conditions. The mean migration distance of NG2 cells in the proximal quadrant of the explant was normalized with that in the distal quadrant of the same explant. Data were analyzed from the same explants as shown in D. **, P < 0.01 compared with control group. (F) Averaged ratio (proximal/distal quadrants) of the total migration distance of NG2 cells from the edge of SVZ explants under various conditions. The summed migration distance of all the NG2 cells in the proximal quadrant of the explant was normalized with that in the distal quadrant of the same explant. Data were analyzed from the same explants as shown in D. **, P < 0.01 compared with the control group. Error bars represent mean ± SEM.

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