ORP1L controls cholesterol-dependent LE positioning. (A) Cholesterol-dependent ORP1L vesicle positioning. Mel JuSo cells expressing mRFP-ORP1L were cultured under control conditions (FCS) or conditions causing decreased (statin) or increased (U-18666A) cholesterol levels. Actin was stained with falloidin (green) to mark the cell boundaries before analyses by CLSM. n > 100. (B) ORP1L is dominant in vesicle repositioning as the result of cholesterol manipulations. Mel JuSo cells expressing mRFP-ΔORDPHDPHD or mRFP-ΔORD were treated and imaged as described in A. n > 100. Bars, 10 µm.