Figure 9.
![Figure 9. Evidence that Inn1 functions downstream of Iqg1 and upstream of Chs2 in AMR-independent cytokinesis. Strains MWY1145 (hof1Δ inn1Δ [pUG36-INN1]; sectors 1–3), MWY764 (hof1Δ iqg1Δ [pRS316GW-IQG1]; sectors 7–9), or RNY2225 (hof1Δ chs2Δ [pJC328]; sectors 10–12) were transformed with pRS315GW, pRS315GW-NotI-HOF1, or pRS315GW-C2-HOF1. The resulting transformants and strains MOY632 (hof1Δ innΔ [pUG36-INN1][pRS315GW-C2-HOF1]; sector 4), MOY630 (hof1Δ myo1Δ [pUG36-INN1][pRS315GW-C2-HOF1]; sector 5), and MOY634 (hof1Δ inn1Δ myo1Δ [pUG36-INN1][pRS315GW-C2-HOF1]; sector 6) were streaked on SC-Leu and SC-Leu+FOA plates and incubated at 24°C for 3 d. Vect, vector.](https://cdn.rupress.org/rup/content_public/journal/jcb/185/6/10.1083_jcb.200903125/7/jcb_200903125_gs_fig9.jpeg?Expires=1773794431&Signature=IA1oNVXIhGQJxuk7ygYScnNIP~LB~mvxSkbZ4ax2duFRheERTgBBsbgIvjVooYTL4jqori7lmWTMmey3S2n9peV169RFGcLU6x5YbXpK~4f-Ln5hv8UO1onAiCEh35LjCUylh0P3wJFgB5If8SWR~utUd7jOSbpPewtTR48bcUGBGrPXEQC6k78xNg0PLibOCS8EXQde2MatigNoRh6LN43Z83HRnMejrkWc4JXXEgLMoKTqYzCdpOBFwfP7lPkdw5TYkBN42RsX-hbH5Kz7yPSbRjC3mYKdfyUKmgfoPhDqvXVv-GqNgwKNRwxhQTs0oUwE3d1R79X~BtxaH5cXDw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Evidence that Inn1 functions downstream of Iqg1 and upstream of Chs2 in AMR-independent cytokinesis. Strains MWY1145 (hof1Δ inn1Δ [pUG36-INN1]; sectors 1–3), MWY764 (hof1Δ iqg1Δ [pRS316GW-IQG1]; sectors 7–9), or RNY2225 (hof1Δ chs2Δ [pJC328]; sectors 10–12) were transformed with pRS315GW, pRS315GW-NotI-HOF1, or pRS315GW-C2-HOF1. The resulting transformants and strains MOY632 (hof1Δ innΔ [pUG36-INN1][pRS315GW-C2-HOF1]; sector 4), MOY630 (hof1Δ myo1Δ [pUG36-INN1][pRS315GW-C2-HOF1]; sector 5), and MOY634 (hof1Δ inn1Δ myo1Δ [pUG36-INN1][pRS315GW-C2-HOF1]; sector 6) were streaked on SC-Leu and SC-Leu+FOA plates and incubated at 24°C for 3 d. Vect, vector.
