![Figure 1. Dependence of PS formation on Inn1. (A) Slow growth of inn1Δ cells. Wild-type (WT; YEF473A) and inn1Δ (YEF5216) cells were streaked on an SC plate and incubated at 25°C for 3 d. (B) Abnormal but complete cytokinesis in inn1Δ cells. YEF5216 cells carrying plasmid pRS315-GFP-RAS2 were grown to exponential phase in SC-Leu liquid medium at 23°C and imaged by DIC and fluorescence microscopy. 1 and 2 indicate bud necks. (C) Absence of PS formation in inn1Δ cells. Strains YEF473A and YEF5216 were grown to exponential phase in SC medium at 24°C and examined by TEM. CW, cell wall; PM, plasma membrane. (D) Localization of Chs2 to the neck in inn1Δ cells. Strain LY1373 (inn1Δ CHS2-GFP [pUG36-INN1]) was transferred from an SC plate to an SC+FOA plate, incubated overnight at 25°C to select for loss of the URA3-marked INN1 plasmid, and examined by fluorescence microscopy. (E) Abnormal contraction of the AMR in inn1Δ cells. inn1Δ MYO1-GFP cells (YEF5291) were observed by time-lapse microscopy. Cell bodies are outlined in the GFP panels.](https://cdn.rupress.org/rup/content_public/journal/jcb/185/6/10.1083_jcb.200903125/7/jcb_200903125_gs_fig1.jpeg?Expires=1773794430&Signature=aCZhcmDNqDBhPsBMdAzJFBV9MgvnhNbCx50dl1ToKnm15KHA5Gc4qiSBBHe5LbNwZgCatZjzdStAwIBREghpjyx5rY2xIQhFbuIfYqE5nLtXuD2W0QkrL8X0HbV4VyJfd~YjG3IRNU-JjsSPT6f83dTh7bbKTVqGK~ub5py5zshgAdks~Ri8P~IACXcKTGKBoULGqt49l6oEfpmZDiqABZ5sATry11TUaf6tIbZhBVkdpp0dlajm37AqQm2tJxWRgnLyPIbD4tyxPT7RKUlyDDfC38lzdqJTPehQR5ajsyJjZKI7yR3sSvQj-fgh~uI8csy0jjiwcVjtULLSwBKRbw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Dependence of PS formation on Inn1. (A) Slow growth of inn1Δ cells. Wild-type (WT; YEF473A) and inn1Δ (YEF5216) cells were streaked on an SC plate and incubated at 25°C for 3 d. (B) Abnormal but complete cytokinesis in inn1Δ cells. YEF5216 cells carrying plasmid pRS315-GFP-RAS2 were grown to exponential phase in SC-Leu liquid medium at 23°C and imaged by DIC and fluorescence microscopy. 1 and 2 indicate bud necks. (C) Absence of PS formation in inn1Δ cells. Strains YEF473A and YEF5216 were grown to exponential phase in SC medium at 24°C and examined by TEM. CW, cell wall; PM, plasma membrane. (D) Localization of Chs2 to the neck in inn1Δ cells. Strain LY1373 (inn1Δ CHS2-GFP [pUG36-INN1]) was transferred from an SC plate to an SC+FOA plate, incubated overnight at 25°C to select for loss of the URA3-marked INN1 plasmid, and examined by fluorescence microscopy. (E) Abnormal contraction of the AMR in inn1Δ cells. inn1Δ MYO1-GFP cells (YEF5291) were observed by time-lapse microscopy. Cell bodies are outlined in the GFP panels.
