Rho1 specifically regulates AJs but not SJs in formed, remodeling pupal epithelium. (a–a″) Confocal immunofluorescent localization of DE-cadherin (DE-cad; a and a′) and Dlg (a and a″) in Rho1⁷² MARCM clones. Arrowheads identify AJs (a′) and SJs (a″) between clonal cells. (b–b′″) Confocal immunofluorescent localization of DE-cadherin (b–b″) and Dlg (b, b′, and b′″) in apical (b) and lateral (b′–b′″) optical sections of Rho1⁷² MARCM clones. The yellow line (b) identifies where the lateral section (b′–b′″) was taken. The yellow asterisks identify a Rho1⁷² MARCM clone, and the white asterisks identify an analogous nonclonal wild-type cell. Arrows identify AJs (b″) and SJs (b′″) of the Rho1⁷² clonal cell that neighbors another clonal cell on the right and a nonclonal cell on the left. (c–c″) Confocal immunofluorescent localization of DE-cadherin (c and c′) and Dlg (c and c″) in shg^R69 (DE-cad null) MARCM clones. Arrows identify multiple-cell clones, and arrowheads identify single-cell clones. (d–d′″) Confocal immunofluorescent localization of DE-cadherin (d and d″) and Dlg (d and d′″) in pupal wing epithelial cells expressing GFP using patched-gal4. (e–e′″) Confocal immunofluorescent localization of DE-cadherin (e and e″) and Dlg (e and e′″) in pupal wing epithelial cells coexpressing GFP and Rho1-RNAi using patched-gal4. Bars, 10 µm.