Dyn2 is required for the formation of the Golgi complex but not for transport along microtubules. (A) Dyn2 siRNA–treated cells show fragmented Golgi but maintain their stacks. HeLa cells were transfected with the indicated siRNAs and visualized with antibodies specific for GRASP65 (green) and α-tubulin or p230 (red). The boxed areas are enlarged in the insets. (B) Electron micrographs show the Golgi stacks (arrows) in the siRNA-transfected cells. (C) HeLa cells expressing NAGT-GFP were transfected with the indicated siRNAs and treated with nocodazole for 2 h. The cells were then washed and incubated in the absence of nocodazole. Images were collected every 3 s for 30 min. The signals were tracked manually, and representative tracks are shown in different colors. (D and E) The velocity (D) and distance moved (E) by 15 fragments in three cells for each siRNA were measured and averaged. The data are represented in arbitrary units (a.u.). The results represent the mean ± SD. Bars: (A and C) 10 µm; (B) 2 µm.