Expression of the 551Δ3 dyn2 mutant causes the accumulation of acetylated tubulin. (A) COS-7 cells were transfected with the indicated constructs, fixed 48 h later, and visualized with antibodies specific for V5 (red) and acetylated-tubulin (Acet-tubulin; green). (B and C) The expression level of V5-tagged proteins (B) and the level of acetylated tubulin against the V5 signals (C) were measured and averaged. Approximately 50 cells were used, and the data are represented in arbitrary units (a.u.). Error bars indicate SD; *, P < 0.005. WT, wild type. Bar, 20 µm.