Efflux of a nuclear GFP marker accompanies the defective mitosis of cut11.1 and cut12.1 cells at 36°C. (A–D) Wild-type (Wt; A; Video 1), cut7.24 (B; Video 2), cut11.1 (C; Video 3), or cut12.1 (D; Video 4) strains incorporating both integrated NLS-GFP–β-Gal and pRep81Cherry-tubulin plasmid were grown at 25°C and mounted for live imaging at 36°C. Time-lapse images were captured in both green (top image at each time point marker; NLS-GFP–β-Gal nuclear integrity marker) and red (bottom image; tubulin to image microtubules) channels every 4 min. Time 0 indicates the first time lapse in which interphase microtubules are no longer found. GFP signal leaks into the cytoplasm of mitotic (as indicated by the tubulin in the bottom panels) cut12.1 and cut11.1 cells (frames labeled as GFP efflux). No efflux is observed in the wild-type or cut7.24 controls. Bar, 10 µm.