Figure 4.
Figure 4. Transmission electron micrographs of FusMidp-vesicles in different magnifications. (A) Overview of FusMidp-vesicles in an electron micrograph shows the morphological homogeneity of the FusMidp-vesicle population with a diameter of ∼100 nm. These isolated vesicles have the same morphological characteristics as the structures that accumulate in sec6-4 cells at 37°C; see Fig. 2 (B and C). (B) Transmission electron micrograph of a single FusMidp-vesicle. The 100-nm vesicular structure is formed by a membrane whose electron density pattern shows the integrity of the lipid bilayer. (C) A group of FusMidp-vesicles. The lipid bilayer is visible as in B. Bars: (A) 1,000 nm; (B and C) 100 nm.

Transmission electron micrographs of FusMidp-vesicles in different magnifications. (A) Overview of FusMidp-vesicles in an electron micrograph shows the morphological homogeneity of the FusMidp-vesicle population with a diameter of ∼100 nm. These isolated vesicles have the same morphological characteristics as the structures that accumulate in sec6-4 cells at 37°C; see Fig. 2 (B and C). (B) Transmission electron micrograph of a single FusMidp-vesicle. The 100-nm vesicular structure is formed by a membrane whose electron density pattern shows the integrity of the lipid bilayer. (C) A group of FusMidp-vesicles. The lipid bilayer is visible as in B. Bars: (A) 1,000 nm; (B and C) 100 nm.

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