Immunoisolation of FusMidp-vesicles and the TGN/E. (A and B) Several organelle markers were monitored throughout the isolation of FusMidp-vesicles (A) and the TGN/E (B), with GFP detecting the respective baits. InvRFP was used as a specific marker for HDSVs. Gas1p is the major GPI-anchored protein in yeast and is an LDSV marker. Pep12p is the late endosome t-SNARE, Dpm1p is an ER protein, and Tlg1p is a late Golgi marker. Input, sucrose gradient fraction 7 (A) and 4 (B); FT, flow through; Bind, material bound to the immunoadsorbent; Wash, supernatant of the last wash; Remain, material not released from the cellulose fibers after TEV cleavage; ELUATE, eluted material released from the immunoadsorbent after TEV cleavage.