Figure 1.
Figure 1. Intracellular accumulation of the immunoisolation baits at the restrictive temperature 37°C in sec6-4 cells. (A) FusMidGFPLTLM9, the raft-carrier cargo immunoisolation bait, and InvRFP expressed for 45 min. At the permissive temperature 24°C, FusMidGFPLTLM9 reached the PM, whereas InvRFP was secreted and therefore not visible (for InvRFP secretion, see also Fig. S3 B). At the restrictive temperature 37°C, both proteins accumulated intracellularly. (B) Gap1GFPLTLM9, the TGN/E immunoisolation bait, and InvRFP expressed for 45 min. At the permissive temperature 24°C, InvRFP was secreted and, thus, not visible (Fig. S3 B), and Gap1GFPLTLM9 localized to intracellular compartments. At the restrictive temperature 37°C, both proteins accumulated intracellularly. DIC, differential interference contrast. Bars, 2 µm.

Intracellular accumulation of the immunoisolation baits at the restrictive temperature 37°C in sec6-4 cells. (A) FusMidGFPLTLM9, the raft-carrier cargo immunoisolation bait, and InvRFP expressed for 45 min. At the permissive temperature 24°C, FusMidGFPLTLM9 reached the PM, whereas InvRFP was secreted and therefore not visible (for InvRFP secretion, see also Fig. S3 B). At the restrictive temperature 37°C, both proteins accumulated intracellularly. (B) Gap1GFPLTLM9, the TGN/E immunoisolation bait, and InvRFP expressed for 45 min. At the permissive temperature 24°C, InvRFP was secreted and, thus, not visible (Fig. S3 B), and Gap1GFPLTLM9 localized to intracellular compartments. At the restrictive temperature 37°C, both proteins accumulated intracellularly. DIC, differential interference contrast. Bars, 2 µm.

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