Figure 4.
Figure 4. FcRn transports IgG through the MyoVb tail–induced compartment. (a) FcRn partially localizes with the EGFP–MyoVb tail, as assessed in fixed cells stained for FcRn-HA. (b) Time course studies show that IgGs entering cells from apical or basolateral surfaces eventually accumulate in the EGFP–MyoVb tail–induced compartment. Alexa Fluor 568–IgG and Alexa Fluor 647–IgG were added at the apical and basolateral reservoirs, respectively; cells were incubated for 5, 15, 30, or 60 min, then fixed and imaged by confocal microscopy. Objects were defined based on the intensity of the EGFP–MyoVb tail; then, the colocalization of apically applied IgG (ap IgG), basolaterally applied IgG (bs IgG), or both IgGs was measured and quantified as described (see Materials and methods). In the micrographs, the panels on the right show enlarged views of the boxed portions on the left. The horizontal bars in the graphs indicate mean Mtarget protein. Bars, 10 µm.

FcRn transports IgG through the MyoVb tail–induced compartment. (a) FcRn partially localizes with the EGFP–MyoVb tail, as assessed in fixed cells stained for FcRn-HA. (b) Time course studies show that IgGs entering cells from apical or basolateral surfaces eventually accumulate in the EGFP–MyoVb tail–induced compartment. Alexa Fluor 568–IgG and Alexa Fluor 647–IgG were added at the apical and basolateral reservoirs, respectively; cells were incubated for 5, 15, 30, or 60 min, then fixed and imaged by confocal microscopy. Objects were defined based on the intensity of the EGFP–MyoVb tail; then, the colocalization of apically applied IgG (ap IgG), basolaterally applied IgG (bs IgG), or both IgGs was measured and quantified as described (see Materials and methods). In the micrographs, the panels on the right show enlarged views of the boxed portions on the left. The horizontal bars in the graphs indicate mean Mtarget protein. Bars, 10 µm.

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