Cytoplasmic spots containing Nup188 and Nup159 colocalize with the ER marker Sec61 in GAL∷NUP170C-overproducing cells. (A and B) Cells expressing Nup188-CFP (A) and Nup159-CFP (B) in a nup170Δ strain overproducing GAL∷NUP170C were analyzed by fluorescence microscopy. Moreover, these strains coexpressed the ER marker Sec61-YFP. Fluorescence microscopic, merged, and Nomarski pictures of representative cells are shown. Bars, 5 µm.