Figure 2.
Figure 2. Overexpression of GAL∷NUP170C causes a toxic phenotype in nup170Δ cells. (A) Full-length Nup170 and the indicated tagged Nup170C and Nup170N domains were overexpressed in either wild-type (NUP170) or nup170Δ cells. (B) The indicated Nup157N, Nup157C, and Nup170C domains were overexpressed under the control of the GAL promoter in nup170Δ cells. (C) The indicated Nup157C or Nup170C domains were overexpressed under the control of the GAL promoter in nup157Δ cells. (D) Nup170C was expressed either under the control of the GAL promoter or the NOP1 promoter in nup170Δ cells. Cells were spotted in 10-fold serial dilutions on glucose-containing plates (noninducing) and galactose-containing plates (inducing). They were incubated at 30°C for 2 (glucose) and 4 d (galactose). ProtA, protein A.

Overexpression of GAL∷NUP170C causes a toxic phenotype in nup170Δ cells. (A) Full-length Nup170 and the indicated tagged Nup170C and Nup170N domains were overexpressed in either wild-type (NUP170) or nup170Δ cells. (B) The indicated Nup157N, Nup157C, and Nup170C domains were overexpressed under the control of the GAL promoter in nup170Δ cells. (C) The indicated Nup157C or Nup170C domains were overexpressed under the control of the GAL promoter in nup157Δ cells. (D) Nup170C was expressed either under the control of the GAL promoter or the NOP1 promoter in nup170Δ cells. Cells were spotted in 10-fold serial dilutions on glucose-containing plates (noninducing) and galactose-containing plates (inducing). They were incubated at 30°C for 2 (glucose) and 4 d (galactose). ProtA, protein A.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal