Spatial-temporal patterning of CD2, TCR, and signaling molecules. Triple-color imaging of Jurkat cells interacting with bilayer containing fluorescent CD58 and anti-TCR antibody. (A and B) Patterning of CD2–CD58 (green) and TCR (blue) microdomains shows their initial colocalization at 2 min (A) and then separation at 15 min (B) after the initial cell–bilayer contact. Immunofluorescence images of phosphotyrosine (pY) corresponding with these two microdomain patterns are shown at right. (C–E) Localization of signal molecules (active Lck [Y505F]; C), phospho–TCR-ζ (D), and LAT (E) is shown in red in the merged images (separate CD2–CD58 [green] and TCR [blue] microdomains are shown on the left); imaged after 15 min of cell–bilayer contact. Bars, 5 µm.