Figure 7.
Figure 7. A cortactin/Arg fusion protein lacking the SH3–PXXP interaction regions partially rescues adhesion-dependent cell edge protrusion. (A) Graphical depiction of a cortactinΔSH3–Arg 688-C–RFP fusion protein (Fusion) or the same with Tyr 421, 466, and 482 in cortactin mutated to Phe (Fusion 3F). MT, microtubule. (B–E) representative images of 10-min time-lapse videos of cells spreading on glass coverslips covered with fibronectin. The three rightmost panes depict representative kymographs. Kymographs were constructed as in Fig. 2. arg−/− cells expressing the fusion protein (B) and the nonphosphorylatable fusion protein (Fusion 3F; C) and cortactin KD cells expressing the fusion protein (D), or the fusion 3F protein (E) are shown. (F and G) Quantitation of protrusions of either arg−/− cells (F) or cortactin KD cells (G) expressing the indicated constructs. Mean ± SEM; *, P < 0.05. Bars, 10 µm.

A cortactin/Arg fusion protein lacking the SH3–PXXP interaction regions partially rescues adhesion-dependent cell edge protrusion. (A) Graphical depiction of a cortactinΔSH3–Arg 688-C–RFP fusion protein (Fusion) or the same with Tyr 421, 466, and 482 in cortactin mutated to Phe (Fusion 3F). MT, microtubule. (B–E) representative images of 10-min time-lapse videos of cells spreading on glass coverslips covered with fibronectin. The three rightmost panes depict representative kymographs. Kymographs were constructed as in Fig. 2. arg−/− cells expressing the fusion protein (B) and the nonphosphorylatable fusion protein (Fusion 3F; C) and cortactin KD cells expressing the fusion protein (D), or the fusion 3F protein (E) are shown. (F and G) Quantitation of protrusions of either arg−/− cells (F) or cortactin KD cells (G) expressing the indicated constructs. Mean ± SEM; *, P < 0.05. Bars, 10 µm.

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