Ablation of Sam68 affects expression of specific mRNAs in mouse testis. (A) Diagram representing the differences in the transcriptome profile of mouse Sam68^+/+ and Sam68^−/− testes. Three RNA preparations from different animals were used for each sample and independently hybridized on Affymetrix chips. Only tags whose differences gave a P < 0.05 in the t test analysis were reported. Unchanged expressed tags are represented in gray, down-regulated tags in red, and up-regulated tags in green. (B) The top eight functional categories that were significantly enriched among the genes down-regulated (top) or up-regulated (bottom) in the Sam68 knockout testis. Analysis was performed using the IPA Ingenuity Systems. The range displayed corresponds to the most significant functions within high-level functional category. (C) Real-time PCR analysis of 10 of the mRNAs down-regulated in Sam68^−/− testis. Data are represented as the mean ± SD of three separate experiments performed in duplicate.