Cellular localization of Cby protein. (A–F) Nasal epithelial sections from Cby^+/+ (A–C) and Cby^−/− (D–F) adult mice were double stained with antibodies against acetylated α-tubulin (ac-tubulin; A and D) and Cby (B and E), and the merged images of acetylated α-tubulin (red) and Cby (green) are shown (C and F). (G–I) Ciliated MDCK2 cells were doubly immunostained with acetylated α-tubulin (G) and Cby (H) antibodies, and the merged image of acetylated α-tubulin (red) and Cby (green) is shown (I). (J–L) Ciliated MDCK2 cells were double stained with γ-tubulin (J) and Cby (K), and the merged image of γ-tubulin (red) and Cby (green) is shown (L). Arrows show colocalization of Cby with γ-tubulin. (B, C, H, and I) Arrowheads point out Cby staining at the base of the cilia. Bars: (A–F) 6 µm; (G–I) 1 µm; (J–L) 5 µm.