Co-migration of mRFP-Bsn with DLC1-EGFP. Cultured neurons were transfected at 3 DIV with DLC1-EGFP and mRFP-Bsn and observed 16 h later by live imaging. (A) Overview image showing a part of an axon with depicted orientation. The black box marks the region enlarged in B, showing co-migration of DLC1-EGFP (left) and mRFP-Bsn (right). Arrows indicate an anterogradely moving particle, and arrowheads indicate a particle with bidirectional movement. See also Video 1. Bar, 5 µm.