Effects of PDGF-AA and EIPA on translocation of growth-arrested Tg737^orpk MEFs in wound-healing assays. (A) Trajectories (normalized to common starting points) of growth-arrested Tg737^orpk MEFs in the presence of 50 ng/ml PDGF-AA and in the absence or presence of 10 µM EIPA, as indicated. The radii of the red circles illustrate the mean translocation of the cells within a 5-h time period. (B) Translocation (in micrometers) of growth-arrested Tg737^orpk MEFs in the absence or presence of 50 ng/ml PDGF-AA and 10 µM EIPA, as indicated, calculated as described in the legend for Fig. 3. (A and B) Data shown are individual trajectories (A) or means ± SEM (B) of 19–34 cells in two to four independent experiments. (C) Velocity of migration perpendicular to the wound of Tg737^orpk MEFs in the absence (open circles) or presence (closed circles) of EIPA. Data shown are means ± SEM of 19–34 cells in two to four independent experiments. The velocity in the presence of EIPA is significantly different from that in the absence of EIPA (P < 0.0001). Ctrl., control.