Wac is dispensable for somatic mitosis and male meiosis. (A) The genomic organization of the wacΔ mutant. Parentheses represent the deleted region. (B) Immunoblots showing the absence of Wac and Dgt2 proteins in wacΔ adult females. wt, wild type. (C) Increased preanaphase stages in wacΔ. The mitotic index and the frequency of anaphase among mitotic cells in orcein-stained brain squashes with the SD (n = 4). The differences are significant between the homozygous mutant and the control (Ctrl) sibling heterozygotes (P < 0.01). (D) Immunostaining of whole-mount wild-type and wacΔ larval neuroblasts. CP190 is a centrosomal protein. (E) The diameter of spermatid nuclei at the onion stage from wild type and the wacΔ mutant. The distribution is not significantly different between the wild type and mutant (by χ2 test; n > 100). Error bars indicate SD. Bar, 10 µm.
