Expression and localization of Wac and Dgt2 in developing flies. (A) Expression of Wac and Dgt2 proteins during development. E1 and E2 indicate 0–4-h-old and 4–20-h-old embryos, respectively; L1, L2, and L3 indicate first, second, and third instar larvae, respectively; EP and LP indicate early and late darkened pupae, respectively; >, −, and • indicate 16.5 kD, 25 kD, and 55 kD, respectively. (B) Expression of Wac and Dgt2 proteins in adult females. All, whole body of adult females; Tho, thorax; Abd, abdoman. (C) Immunolocalization of Wac and Dgt2 to spindle microtubules in syncytial embryos. Bar, 10 µm.