Modulations of the cell cycle change the nuclear level of active p65 (S276-p65). (A) The nuclear level of S276-p65 was reduced in embryos injected with GemMO, Cdt1 mRNA, or p65MO, whereas it increased in embryos injected with geminin mRNA, Cdt1MO, or MCM5MO. The increased nuclear level of S276-p65 caused by geminin mRNA was rescued by the coinjection of p53MO. (B) The nuclear level of S276-p65 was reduced in embryos with p21MO. α-Tubulin served as a cytoplasmic marker, whereas histone2B served as a nuclear marker and the loading control, indicating a successful separation of nuclear and cytoplasmic phases.