Generation of micropatterned PVA films. (A) Schematic of a single PVA molecule conjugated to a glass coverslip through 3-(amino)propyl-trimethyloxysilane (APTMS) and glutaraldehyde (GA). (B) PVA-coated surfaces (1) are photoablated using an LSM 510 NLO META system (Carl Zeiss, Inc.). (2) ROI template generated by the software dictates the ablation pattern. (3) Protein (red) added to the dish adsorbs only to ablated sites. (C) Phase image of four different ROI templates used in close proximity. (D) Serial ablation with μPP to generate patterns of fibrinogen (green dashes), vitronectin (red squares), and FN (blue circles) within micrometers of each other. The DIC image is shown in the bottom left. Bars: (C) 20 µm; and (D) 5 µm.