![Figure 3. APC/C activation is not required for changes in microtubule stability. (A) CSF extracts containing Cy3-tubulin and in vitro translated exogenously added securin were preincubated for 10 min in the presence of Ran-GTP to preassemble microtubules. Reactions were treated with buffer (Meta), calcium and cyclinBΔ90 (Ana), calcium (Inter), XErpND, or calcium and XErpND (XErpND + Ca2+). Microtubule structures were visualized by direct fluorescence of Cy3-tubulin. (B) Quantification of microtubule assemblies counted at the 40-min time point imaged in A. Error bars represent SD from three independent experiments; metaphase was set to 100%. (C) Amounts of cyclin B (endogenous, X.l. cyclin B; exogenously added [Δ90], H.s. cyclin B) were determined by immunoblotting, and amounts of exogenously added in vitro translated (IVT) securin were determined by autoradiography; Cdk1 activities were measured by histone H1 phosphorylation (pHistone H1). Meta, metaphase; Ana, anaphase; Inter, interphase. Bar, 5 μm.](https://cdn.rupress.org/rup/content_public/journal/jcb/183/6/10.1083_jcb.200807006/8/jcb_200807006_gs_fig3.jpeg?Expires=1771640769&Signature=U-d8cuuXaBNliFY8YAEf2SzT3uA7ulDLY3lpvWVx-EoBzHRiwUJWQK5mm9xNeiWe0iHgCpfN4xkPDqULiRhGiMEFmuEXpoF1oqUtFqTBqjvIjmRBblWD2Yo6hRwuNMv-VB9eEUJaJJLHzlrt~DCqkzQZPJMj-bpBll3oNuOo7wkQ1KvzzJKy2rtvjI7bgPinA4t28rv7UpNs7g0vxbaUrElxNLmiNjkzXfMRU~E0LVTAHLuU6DLxxwVcSbpf3adJq3dzFdFayUkhc7IlLbODN1kCbPWFMyp2gn-660HooNnb7gEGXxgfQ6~Zbt2RqwTyo6Q7hca5WMLPc1k~NhiQ1w__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
APC/C activation is not required for changes in microtubule stability. (A) CSF extracts containing Cy3-tubulin and in vitro translated exogenously added securin were preincubated for 10 min in the presence of Ran-GTP to preassemble microtubules. Reactions were treated with buffer (Meta), calcium and cyclinBΔ90 (Ana), calcium (Inter), XErpND, or calcium and XErpND (XErpND + Ca2+). Microtubule structures were visualized by direct fluorescence of Cy3-tubulin. (B) Quantification of microtubule assemblies counted at the 40-min time point imaged in A. Error bars represent SD from three independent experiments; metaphase was set to 100%. (C) Amounts of cyclin B (endogenous, X.l. cyclin B; exogenously added [Δ90], H.s. cyclin B) were determined by immunoblotting, and amounts of exogenously added in vitro translated (IVT) securin were determined by autoradiography; Cdk1 activities were measured by histone H1 phosphorylation (pHistone H1). Meta, metaphase; Ana, anaphase; Inter, interphase. Bar, 5 μm.
