Figure 5.
Figure 5. Nce102 is homogenously distributed in membranes of buds and shmoos. (A) Development of the membrane distribution of Nce102-GFP, Sur7-GFP, and Pil1-GFP in mother cells and buds of increasing size (I–IV). (B) Localization of the proteins examined in A and Can1-GFP in cells (genetic background: BY4741 MATa; OD600 = 0.25) treated with 30 μg/ml α factor for 2 h. 3D reconstructions of confocal z stacks are presented. Bars, 2 μm.

Nce102 is homogenously distributed in membranes of buds and shmoos. (A) Development of the membrane distribution of Nce102-GFP, Sur7-GFP, and Pil1-GFP in mother cells and buds of increasing size (I–IV). (B) Localization of the proteins examined in A and Can1-GFP in cells (genetic background: BY4741 MATa; OD600 = 0.25) treated with 30 μg/ml α factor for 2 h. 3D reconstructions of confocal z stacks are presented. Bars, 2 μm.

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