Nce102 is required for MCC localization of Can1. Plasma membrane distribution of Can1-GFP was observed in nce102Δ cells expressing Nce102-mRFP under the control of a galactose-inducible promoter. After the induction, gradual restoration of the wild type–like patchy distribution of Can1-GFP was followed on tangential confocal sections. On transversal sections of the same cells, it is notable that the pattern of bud membrane was restored earlier (arrowheads). Bar, 5 μm.