Efr3 recruits PIK patch components to the PM. (A) Growth assay of EFR3 wild type and efr3-1 temperature-sensitive yeast at 26 and 38°C. (B) Phosphoinositide levels of EFR3 and efr3-1 at 38°C. Data are presented as means and SD (error bars) of two independent experiments. (C) Cellular localization of the PtdIns4P reporter GFP-2xPH^Osh2 in efr3-1 yeast at the permissive (top) and restrictive (bottom) temperatures. Bars, 4 μm. (D) The localization profile of tet^o-EFR3 GFP-Stt4 in the absence (top) or presence (bottom) of doxycycline and the corresponding DIC image. Arrows indicate PIK patch localization. Bars, 4 μm. (E) The localization profile of tet^o-YPP1 Efr3-GFP in the absence (top) or presence (bottom) of doxycycline and the corresponding DIC images. Arrows indicate PIK patch localization. Bars, 4 μm.