Stt4 and Ypp1 are codependent for proper localization and organization into PIK patches at the PM. (A) Relative abundance of GFP-Stt4 after doxycycline treatment in tet^o-YPP1 cells over the time course of 16 h. The relative abundance of G6PDH is shown as a control. (B) The localization profile of tet^o-YPP1 GFP-Stt4 in the absence (top) or presence (second panel) of doxycycline and the corresponding DIC image. The localization tet^o-STT4 GFP-Ypp1 in the absence (third panel) or presence (bottom) of doxycycline and the corresponding DIC images. Bars, 4 μm. (C) Localization of PM anchored Psr1^1-28-GFP-Ypp1 expressed under wild-type conditions (left) or in the sac1Δ stt4Δ background (right). The graph is the relative fluorescence intensity along the PM within the indicated spatial region. Arrows indicating the start and end of the fluorescent images correspond to the x axis of the graph.