Deletion of SAC1 bypasses ypp1Δ or stt4Δ. (A) Relative growth profiles of wild type, sac1Δ, sac1Δ stt4Δ, sac1Δ ypp1Δ, stt4Δ, and ypp1Δ at 26°C. (B) Phosphoinositide levels of wild type, sac1Δ, sac1Δ stt4Δ, and sac1Δ ypp1Δ. Data are presented as means and SD (error bars) of three independent experiments. (C) Cellular localization of the PtdIns4P reporter GFP-2xPH^Osh2 in wild type, pik1^ts, and stt4^ts (top) at the restrictive temperature. (bottom) The fluorescent localization of GFP-2xPH^Osh2 in tet^o-YPP1 grown in the presence (bottom) or absence (top) of doxycycline for 20 h. Bars, 4 μm.