Figure 4.
Figure 4. ACA vesicles are not targeted to lysosomes and require proper assembly of clathrin for trafficking. (A) Montage of confocal fluorescent images depicting Dextran–Alexa 568 (red) and ACA-YFP (green) distribution in ACA-YFP/aca− cells. (B) Spinning disk and confocal fluorescent images of ACA-YFP/aca− cells labeled with FM4-64 (red) for 1 and 30 min and ACA-YFP (green). In the top six images, maximum intensity projections are presented. The bottom two images represent confocal slices taken from the maximum intensity projection of the merge. (C) Images of clc− and chc− cells chemotaxing to a micropipette containing 1 μM cAMP. (D) Confocal fluorescent images of ACA-YFP/aca− and ACA-U-YFP/clc− cells colabeled with lysotracker red. The asterisk represents the position of the micropipette.

ACA vesicles are not targeted to lysosomes and require proper assembly of clathrin for trafficking. (A) Montage of confocal fluorescent images depicting Dextran–Alexa 568 (red) and ACA-YFP (green) distribution in ACA-YFP/aca− cells. (B) Spinning disk and confocal fluorescent images of ACA-YFP/aca− cells labeled with FM4-64 (red) for 1 and 30 min and ACA-YFP (green). In the top six images, maximum intensity projections are presented. The bottom two images represent confocal slices taken from the maximum intensity projection of the merge. (C) Images of clc− and chc− cells chemotaxing to a micropipette containing 1 μM cAMP. (D) Confocal fluorescent images of ACA-YFP/aca− and ACA-U-YFP/clc− cells colabeled with lysotracker red. The asterisk represents the position of the micropipette.

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