Figure 4.
Figure 4. FAM29A recruits NEDD1 onto spindle MTs. (A and B) Maximum projections from deconvolved z stacks of representative HeLa or HeLa/GFP-FAM29A cells transfected with siRNAs and stained for NEDD1 (green), β-tubulin (red), and DNA (blue) (A). NEDD1 fluorescence intensity on spindle (excluding spindle pole signals) was quantified (B; n = 10 metaphase cells). *, P < 0.01 (two-tailed t test relative to siControl metaphase cells). (C and D) Maximum projections from deconvolved z stacks of representative HeLa cells transfected with siRNAs and stained for FAM29A (green), NEDD1 (C)/γ-tubulin (D) (red), and DNA (blue). Error bars, SEM. Bars, 5 μm.

FAM29A recruits NEDD1 onto spindle MTs. (A and B) Maximum projections from deconvolved z stacks of representative HeLa or HeLa/GFP-FAM29A cells transfected with siRNAs and stained for NEDD1 (green), β-tubulin (red), and DNA (blue) (A). NEDD1 fluorescence intensity on spindle (excluding spindle pole signals) was quantified (B; n = 10 metaphase cells). *, P < 0.01 (two-tailed t test relative to siControl metaphase cells). (C and D) Maximum projections from deconvolved z stacks of representative HeLa cells transfected with siRNAs and stained for FAM29A (green), NEDD1 (C)/γ-tubulin (D) (red), and DNA (blue). Error bars, SEM. Bars, 5 μm.

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