New model for regulation of cytoplasmic dynein at kinetochores. During prometaphase, dynein is loaded onto kinetochores via binding of phosphodynein to zw10 (t = 1). As MTs attach to kinetochores and chromosomes congress to the metaphase plate (t = 2), dynein undergoes dephosphorylation, shifting binding from zw10 to dynactin (t = 2). This transition stimulates dynein for translocation away from kinetochores (t = 3). Because activated dynein contributes to the removal of anaphase inhibitors from kinetochores, dynein dephosphorylation reduces the ability of kinetochores to produce active anaphase inhibitors.