Impact of p50(dynamitin) expression on PT89-dynein localization. (A) Control (1–4) and p50(dynamitin)-expressing (5–8) NRK2 cells stained for chromatin (DAPI; 1 and 5), total dynein ICs (V3; 2 and 6), and BubR1 (3 and 7) reveal a colocalization of total dynein with BubR1 on kinetochores during prometaphase in controls (4, inset) but a depletion of total dynein from prometaphase kinetochores in cells expressing moderate levels of p50(dynamitin) (8, inset). Intensity scales in panels 2 and 6 correspond to levels from 0 to 800 intensity units. (B) Nocodazole-treated control (1–4) and p50(dynamitin)-expressing (5–8) NRK2 cells stained for chromatin (DAPI; 1 and 5), p150Glued (2 and 6), and PT89-dynein (3 and 7) reveal similar levels of PT89-dynein on kinetochores with normal (2 and 4) or depleted (6 and 8) levels of p150Glued. Bars, 5 μm.
