Figure 5.
Figure 5. Mutations that inhibit P-body formation also inhibit stress granule assembly. (A) Various yeast deletion strains and wild-type isogenic controls were transformed with pRP1657, pRP1658, or pRP1659, according to auxotrophies and genetic properties of the strains; bottom three panels feature Edc3-mCh as a P-body marker, others feature Dcp2-mCh. Glucose deprivation and quantitation as shown in Fig. 3. (B) Decapping (dcp1Δ) and 5′–3′decay (xrn1Δ) mutant strains were transformed with pRP1659. (C) Individual Pab1-GFP and Edc3-mCh images from +glucose xrn1Δ control cells, highlighting large and diffuse nature of constitutive Edc3-mCh foci (more visible at 4x scaling intensity) and occasional large and diffuse Pab1-GFP foci. (D) Zoom panels from dcp1Δ images in B (white boxes), showing examples of Pab1-GFP forming donut-like foci, with Edc3 foci overlapping the Pab1-GFP holes.

Mutations that inhibit P-body formation also inhibit stress granule assembly. (A) Various yeast deletion strains and wild-type isogenic controls were transformed with pRP1657, pRP1658, or pRP1659, according to auxotrophies and genetic properties of the strains; bottom three panels feature Edc3-mCh as a P-body marker, others feature Dcp2-mCh. Glucose deprivation and quantitation as shown in Fig. 3. (B) Decapping (dcp1Δ) and 5′–3′decay (xrn1Δ) mutant strains were transformed with pRP1659. (C) Individual Pab1-GFP and Edc3-mCh images from +glucose xrn1Δ control cells, highlighting large and diffuse nature of constitutive Edc3-mCh foci (more visible at 4x scaling intensity) and occasional large and diffuse Pab1-GFP foci. (D) Zoom panels from dcp1Δ images in B (white boxes), showing examples of Pab1-GFP forming donut-like foci, with Edc3 foci overlapping the Pab1-GFP holes.

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